component 1: Orientation of photos in the MicroscopePart 2: practice with Depth of ar in the MicroscopePart 3: investigation of Pond Water & microbe

(Adapted from

Parts that the Microscope:



Your microscope has actually 4 target lenses: Scanning (4x), low (10x), High (40x), and Oil Immersion (100x). In this lab, you will not use the oil immersion lens; the is because that viewing microorganisms and requires technological instructions not covered in this procedure.

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In addition to the objective lenses, the ocular lens (eyepiece) has a magnification. The complete magnification is figured out by multiply the magnification the the ocular and also objective lenses.

Magnification Ocular Lens Total Magnification
Scanning 4x 10x 40x
Low Power 10x 10x 100x
High Power 40x 10x 400x
Oil Immersion 100x 10x 1000x

General Procedures:

1. Make sure all backpacks, purses, etc. Room off the benchtop.

2. Lug the microscope by the base and arm through both hands.

3. Save with the cord wrapped about the microscope and the scanning objective clicked into place.

Focusing Specimens:

1. Plug your microscope right into the power supply and switch top top the illuminator.

2. Always start through the phase as low as possible and using scanning target (4x). Odds are, girlfriend will have the ability to see something on this setting (sometimes it’s just a color). Use the rough circuit knob to focus: the photo may be tiny at this magnification, yet you won"t have the ability to find it on the higher powers without this an initial step. Relocate the mechanical stage until your concentrated image is also centered.

3. As soon as you"ve concentrated using the scanning objective, switch to the low strength objective (10x). Usage the outlet knob come refocus and move the mechanical stage to re-center her image. Again, if friend haven"t concentrated on this level, you will certainly not be able to move to the next level.

4. Currently switch to the high power objective (40x). In ~ this point, only use the fine adjustment knob to focus specimens.

5. If the specimen is too light or too dark, try adjusting the diaphragm.


1. Store microscope with the scanning target in place and also the stage in its shortest position.

2. Pave cords roughly the microscope.

3. Change slides to original slide tray.


Compound microscope Microscope slide v the letter “e”


Compound microscopic lense Microscope slide with 3 threads


Compound microscope Microscope slide Coverslip deliver pipette Pond water sample


1. Utilizing the carry pipette, move a fall of pond water top top a microscope slide. The ideal specimens normally come native the bottom and also probably will certainly contain chunks of algae or various other debris the you have the right to see with your nude eye.

2. Place the coverslip onto the slide.

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3. Usage the SCANNING (4x) target to focus, then move the mechanically stage around to scan the slide because that live microorganisms. Friend are in search of tiny swim beings- they may look green or clear and also might be very small. Select one to emphasis on and center it in your visual field.


You may wish to use the ProtoSlo to store your bsci-ch.orglogy from swimming as well quickly!